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Journal / Immunology and Infection / Alstrande
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Immunology and Infection

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Journal (Immunology and Infection)
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Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
Journal (Immunology and Infection)
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Alstrande

Article DOI: 10.3791/54697-v • 08:48 min • October 25th, 2016
October 25th, 2016 •
1,2, *2, *2, 2, 1,2, 1,2, 2, 2, 1,2, 1,2
1Department of Immunology, University of Toronto, 2Princess Margaret Cancer Centre, University Health Network
* These authors contributed equally

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Häri beskriver vi en ny metod för att generera antigenspecifika T-cellreceptorer (TCR) genom att para ihop den TCRα eller TCRβ av en befintlig TCR, som har den antigenspecificitet av intresse, med komplementär hemichain av den perifera T-cell-receptorrepertoar. De novo genererade TCRs behålla antigen-specificitet med varierande affinitet.

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Immunologi T-cell antigenpresenterande cell T-cellreceptor hemichain retroviral transduktion antigenspecificitet människa kloning
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