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Tre-dimensionel Super Resolution Mikroskopi af F-actinfilamenter af Interferometrisk fotoaktiverede Lokalisering Microscopy (iPALM)

Article DOI: 10.3791/54774-v • 11:57 min • December 1st, 2016
December 1st, 2016 •
1, 2,3
1Department of Biology, South University of Science and Technology of China, Shenzhen, 2Mechanobiology Institute, Singapore, 3Department of Biomedical Engineering, National University of Singapore

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Vi præsenterer en protokol for anvendelse af interferometrisk fotoaktiverede Lokalisering Microscopy (iPALM), en 3-dimensionel single-molekyle lokalisering super opløsning mikroskopi metode til billeddannelse af actincytoskelettet i vedhængende pattedyrceller. Denne fremgangsmåde giver lys-baserede visualisering af nanostørrelse strukturelle træk, der ellers ville forblive uløste ved konventionel diffraktionsbegrænset optisk mikroskopi.

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Biofysik Super opløsning mikroskopi PALM iPALM Fluorescens F-actin Single Molecule Lokalisering Photoswitchable Fluoroforer interferometri
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