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Journal / Immunology and Infection / Dextran erhöht die Effizienz der Lentivirale…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Dextran erhöht die Effizienz der Lentivirale Transduktion von murinen und menschliche primäre NK-Zellen
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Dextran erhöht die Effizienz der Lentivirale Transduktion von murinen und menschliche primäre NK-Zellen

Article DOI: 10.3791/55063-v • 08:42 min • January 15th, 2018
January 15th, 2018 •
1, 2, 3, 4, 5, 6, 6, 1,5,6,7
1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, The Blood Center of Wisconsin, 2Vector Core Lab, Blood Research Institute, The Blood Center of Wisconsin, 3Laboratory of Lymphocyte Biology, Blood Research Institute, The Blood Center of Wisconsin, 4Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute, The Blood Center of Wisconsin, 5Department of Microbiology and Immunology, The Medical College of Wisconsin, 6Department of Pediatrics, The Medical College of Wisconsin, 7Department of Medicine, The Medical College of Wisconsin

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Das Ziel dieser Studie war es, Technologien zu formulieren, die für erfolgreiche gen Transduktion in primären natürlichen killer (NK) Zellen ermöglichen. Die Dextran-vermittelten Lentivirale Transduktion von Mensch oder Maus primären NK Zellen führt zu höheren Wirkungsgraden der gen-Expression. Diese Methode des Gens Transduktion wird NK Zelle genetischen Manipulation erheblich verbessern.

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Frage 131 Transduktion primäre NK-Zellen Dextran Immunologie Lentivirus gentechnisch veränderte Immuntherapie
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