Waiting
登录处理中...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Dextran Enhances the Lentiviral Transduction…
JoVE Journal
Immunology and Infection

需要订阅 JoVE 才能查看此 内容.

JoVE Journal Immunology and Infection
Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells
High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection
Journal (Immunology and Infection)
High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection
Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus
Journal (Immunology and Infection)
Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus
 

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells

Article DOI: 10.3791/55063-v • 08:42 min • January 15th, 2018
January 15th, 2018 •
1, 2, 3, 4, 5, 6, 6, 1,5,6,7
1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, The Blood Center of Wisconsin, 2Vector Core Lab, Blood Research Institute, The Blood Center of Wisconsin, 3Laboratory of Lymphocyte Biology, Blood Research Institute, The Blood Center of Wisconsin, 4Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute, The Blood Center of Wisconsin, 5Department of Microbiology and Immunology, The Medical College of Wisconsin, 6Department of Pediatrics, The Medical College of Wisconsin, 7Department of Medicine, The Medical College of Wisconsin

章节

总结概括
Automatic Translation

The goal of this study was to formulate technologies that allow for successful gene transduction in primary natural killer (NK) cells. The dextran-mediated lentiviral transduction of human or mouse primary NK cells results in higher gene expression efficiencies. This method of gene transduction will vastly improve NK cell genetic manipulation.

Tags

Dextran Lentiviral Transduction Primary NK Cells Gene Transduction Immunotherapy Genetic Modification Efficiency Gene Expression Lentiviral Vectors Purifying NK Cells Interleukin-2 RPMI1640 Complete Medium Non-adherent Population Flow Cytometry MK Cell-specific Markers Purity Peripheral Blood Mononuclear Cells (PBMCs) Density Gradient
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter