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使用高含量显微镜的细胞氧化还原分析
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使用高含量显微镜的细胞氧化还原分析

Article DOI: 10.3791/55449-v • 11:37 min • May 14th, 2017
May 14th, 2017 •
1,2, 1,2, 1, 3, 1,2
1Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, 2Cell Systems and Imaging Research Group (CSI), Department of Molecular Biotechnology, Ghent University, 3Department of Biochemistry , Radboud Institute for Molecular Life Sciences, Radboud University Medical Center

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本文提出了一种高含量显微镜工作流程，用于同时定量细胞内ROS水平，以及线粒体膜电位和形态 - 共同称为线粒体形态功能 - 使用细胞透过性荧光报告分子5-（和 - 6） - 氯甲基-2'，7'-二氯二氢荧光素二乙酸酯，乙酰酯（CM-H 2 DCFDA）和四甲基罗丹明甲酯（TMRM）。

Tags

细胞生物学，第123期，活性氧物质，线粒体，线粒体形态，活细胞成像，高含量显微镜，荧光显微镜，定量多参数显微镜，氧化还原生物学
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