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In Vitro Ovule dyrkning for Live-celle Imaging af Zygote polarisering og Embryo mønstre i Arabidopsis thaliana

Article DOI: 10.3791/55975-v • 05:41 min • September 11th, 2017
September 11th, 2017 •
*1,2, *1, 1,2,3, 1,3
1Division of Biological Science, Graduate School of Science, Nagoya University, 2Higashiyama Live-Holonics Project, JST-ERATO, Nagoya University, 3Institute of Transformative Bio-Molecules (ITbM), Nagoya University
* These authors contributed equally

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Dette manuskript beskriver en in vitro- ovule dyrkning metode, der muliggør live-celle billeddannelse af Arabidopsis zygotes og embryoner. Denne metode er udnyttet til at visualisere den intracellulære dynamics under zygote polarisering og celle skæbne specifikation i udviklingen af embryoner.

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Udviklingsmæssige biologi sag 127 Plantebiologi befrugtede æg embryoner live-celle imaging Arabidopsis thaliana
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