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Journal / Developmental Biology / Visualizing Multiciliated Cells in the Zebrafish…
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Developmental Biology

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JoVE Journal Developmental Biology
Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
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Journal (Developmental Biology)
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Single-cell Photoconversion in Living Intact Zebrafish
Journal (Developmental Biology)
Single-cell Photoconversion in Living Intact Zebrafish
 

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Article DOI: 10.3791/56261-v • 09:33 min • November 18th, 2017
November 18th, 2017 •
1, 1, 1, 1, 1
1Department of Biological Sciences, University of Notre Dame

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Cilia development is vital to proper organogenesis. This protocol describes an optimized method to label and visualize ciliated cells of the zebrafish.

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Visualizing Multiciliated Cells Zebrafish Protocol Whole Mount Fluorescent In Situ Hybridization Immunofluorescence Developmental Biology Multiciliated Sulfates Embryonic Zebrafish Kidney Protein Labeling RNA Labeling Zebrafish-specific Antibodies Fixing Paraformaldehyde Washing Methanol Incubation Rehydration
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