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Evaluering af celle Invasion og migrationsprocessen: en sammenligning af den Video mikroskop-baseret skrabespil sår Assay og Boyden kammer Assay

Article DOI: 10.3791/56337-v • 11:20 min • November 17th, 2017
November 17th, 2017 •
*1,2, *1,2, 1,2, 1, 1,3, 1,3, 1,3, 1,2, 1,3, 1,2
1UMR CNRS 5822 /IN2P3, IPNL, PRISME, Laboratoire de Radiobiologie Cellulaire et Moléculaire, Faculté de Médecine Lyon-Sud, Université Lyon 1, 2Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, 3Hospices Civils de Lyon, Centre Hospitalier Lyon-Sud
* These authors contributed equally

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Denne undersøgelse rapporter to forskellige metoder til analyse af celle invasion og migration: Boyden kammer assay og in vitro- video mikroskop-baserede sårheling analysen. Protokoller for disse to eksperimenter er beskrevet, og deres fordele og ulemper sammenlignes.

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Kræftforskning sag 129 Boyden kammer Scratch sår Invasion/migration celle motilitet Video-mikroskopi celle chemotaxis
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