共聚焦显微镜通过图像相关光谱法揭示细胞表面受体聚集现象
6.9K 次观看
•
被引用 5 次
•
06:51 分钟
•
2018年8月2日
DOI :10.3791/57164-v
2018年8月2日
•6.9K 次观看
1Tumour Targeting Laboratory, Olivia Newton-John Cancer Research Institute, 2School of Cancer Medicine, La Trobe University, 3Centre for Micro-Photonics, Faculty of Science, Engineering and Technology, Swinburne University of Technology, 4LIMS Bioimaging Facility, La Trobe Institute for Molecular Science, La Trobe University, 5Department of Medical Oncology, Olivia Newton-John Cancer and Wellnes Centre, Austin Health, 6Department of Medicine, University of Melbourne, 7Department of Molecular Imaging and Therapy, Austin Health
结合细胞表面靶受体的抗体可引起构象变化和受体聚集。这些动态变化对于表征靶细胞中药物开发具有重要意义。本方案利用共聚焦显微镜结合ImageJ/FIJI中的图像相关光谱学方法，定量分析细胞表面受体聚集的程度。
Chapters in this video
0:04
Title
0:39
Calculation of Image Correlation Spectroscopy
3:43
Batch Processing of ICS Datasets and Calculation of the Microscope s Beam Area
4:59
Results: Image Correlation Spectroscopy Detects Clusters on the Cell Surface of Adherent Cancer Cells
5:55
Conclusion
Related Videos
版权所有 © 2026 MyJoVE Corporation。保留所有权利。