成人脑组织切片中钾离子选择性电极的制备、检测及应用
12,052 Views
•
11:20 min
•
May 07, 2018
DOI:
Summary
Automatically generated
钾离子有助于细胞的静止膜电位, 胞外 K+浓度是细胞兴奋性的重要调节因子。我们描述如何制作, 校准和使用单 K+选择性电极。使用此类电极可以测量成年海马切片中电诱发的 K+浓度动态。
Related Videos
10:29
Whole Cell Recording from an Organotypic Slice Preparation of Neocortex
Related Videos
14177 Views
09:59
GABA-activated Single-channel and Tonic Currents in Rat Brain Slices
Related Videos
25191 Views
08:46
Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature
Related Videos
26571 Views
08:08
Real-time Electrophysiology: Using Closed-loop Protocols to Probe Neuronal Dynamics and Beyond
Related Videos
11455 Views
11:08
Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue
Related Videos
13553 Views
10:53
Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method
Related Videos
46258 Views
07:23
Whole-cell Patch-clamp Recordings in Brain Slices
Related Videos
59816 Views
11:56
Slice Patch Clamp Technique for Analyzing Learning-Induced Plasticity
Related Videos
15334 Views
07:27
Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices
Related Videos
9859 Views
01:28
Characterizing Extracellular Space in a Brain Slice Using Ion-Selective and Iontophoresis Microelectrodes
Related Videos
86 Views
Read Article
Cite this Article
Octeau, J. C., Faas, G., Mody, I., Khakh, B. S. Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain. J. Vis. Exp. (135), e57511, doi:10.3791/57511 (2018).
Copy