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Laktat Dehydrogenase Sequestration Assay — En simpel og pålidelig metode til at bestemme Bulk Autophagic beslaglæggelse aktivitet i pattedyrceller

Article DOI: 10.3791/57971-v • 09:34 min • July 27th, 2018
July 27th, 2018 •
*1, *1, 1
1The Autophagy Team, Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, University of Oslo
* These authors contributed equally

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Her beskrives et simpelt og godt valideret protokol til måling af bulk autophagic beslaglæggelse aktivitet i pattedyrsceller. Metoden er baseret på kvantificere andelen af laktat dehydrogenase (LDH) i sedimentable celle fraktioner i forhold til samlede cellulære LDH niveauer.

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Biologi sag 137 LDH laktat dehydrogenase binding autophagy autophagic gods phagophore autophagosome Bafilomycin A1 LC3
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