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Journal / Genetics / Endogen Protein taggning i mänskliga inducerade…
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Genetics
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JoVE Journal Genetics
Endogen Protein taggning i mänskliga inducerade pluripotenta stamceller med CRISPR/Cas9
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Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach
 
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Endogen Protein taggning i mänskliga inducerade pluripotenta stamceller med CRISPR/Cas9

Article DOI: 10.3791/58130-v • 14:49 min • August 25th, 2018
August 25th, 2018 •
1, 1, 1, 1, 1, 1
1Allen Institute for Cell Science

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Beskrivs här är ett protokoll för taggning endogenously uttryckte proteiner med fluorescerande Taggar i mänskliga inducerade pluripotenta stamceller med CRISPR/Cas9. Förment redigerade celler berikas av fluorescens aktiverad cell sortering och klonal cellinjer genereras.

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Genetik fråga 138 stamceller hiPSC molekylärbiologi CRISPR Cas9 genomet Engineering Gene Knock-in Gene Tagging fluorescerande Protein
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