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JoVE Journal Chemistry
Store ovenfra og ned Proteomikk bruker kapillær sone geleelektroforese Tandem massespektrometri
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Store ovenfra og ned Proteomikk bruker kapillær sone geleelektroforese Tandem massespektrometri

Article DOI: 10.3791/58644-v • 10:05 min • October 24th, 2018
October 24th, 2018 •
*1, *1, 1, 2, 2,3, 1
1Department of Chemistry, Michigan State University, 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, 3Center for Computational Biology and Bioinformatics, Indiana University School of Medicine
* These authors contributed equally

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En detaljert protokoll er beskrevet for separasjon, identifikasjon og karakterisering av proteoforms i protein prøver ved kapillær sone geleelektroforese-electrospray ionization-tandem massespektrometri (CZE-ESI-MS/MS). Protokollen kan brukes for høy oppløsning karakterisering av proteoforms i enkle protein prøver og store identifikasjonen av proteoforms i komplekse proteom prøver.

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Kjemi problemet 140 ovenfra og ned Proteomikk kapillære sone geleelektroforese electrospray ionization tandem massespektrometri proteoform Escherichia coli
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