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Groß angelegte Top-Down-Proteomik mit Kapillare Zone Elektrophorese Tandem-Massenspektrometrie
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Groß angelegte Top-Down-Proteomik mit Kapillare Zone Elektrophorese Tandem-Massenspektrometrie

Article DOI: 10.3791/58644-v • 10:05 min • October 24th, 2018
October 24th, 2018 •
*1, *1, 1, 2, 2,3, 1
1Department of Chemistry, Michigan State University, 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, 3Center for Computational Biology and Bioinformatics, Indiana University School of Medicine
* These authors contributed equally

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Ein detailliertes Protokoll wird für die Trennung, Identifikation und Charakterisierung von Proteoforms in Proteinproben mit Kapillare Zone Elektrophorese-Electrospray Ionisierung-Tandem-Massenspektrometrie (CZE-ESI-MS/MS) beschrieben. Das Protokoll kann für die hochauflösende Charakterisierung von Proteoforms in einfachen Proteinproben und der großen Identifikation von Proteoforms in komplexen Proteom Proben verwendet werden.

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Chemie Ausgabe 140 Top-Down-Proteomics Kapillare Zone Elektrophorese Elektrospray-Ionisation Tandem-Massenspektrometrie Proteoform Escherichia coli
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