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JoVE Journal Genetics
Karakterisering van Histone posttranslationele wijziging wijzigingen in gist neurodegeneratieve Proteinopathy modellen
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Karakterisering van Histone posttranslationele wijziging wijzigingen in gist neurodegeneratieve Proteinopathy modellen

Article DOI: 10.3791/59104-v • 08:33 min • March 24th, 2019
March 24th, 2019 •
1,2, 1,3, 1, 1, 1, 1, 1,4
1Chemistry Department, Brooklyn College, 2Ph.D. Program in Biochemistry, Graduate Center of the City University of New York, 3Ph.D. Program in Chemistry, Graduate Center of the City University of New York, 4Ph.D. Programs in Chemistry, Biochemistry, and Biology, Graduate Center of the City University of New York

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Dit protocol beschrijft experimentele procedures karakteriseren van genoom-brede veranderingen in de niveaus van Histon posttranslationele modificaties (PTM) die zich voordoen in verband met de overexpressie van eiwitten ALS en Parkinson in zijn gekoppeld Saccharomyces cerevisiae modellen. Na SDS-pagina de scheiding, worden individuele Histon PTM niveaus gedetecteerd met wijziging-specifieke antilichamen via het westelijke bevlekken.

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Genetica kwestie 145 neurodegeneratie Amyotrofische laterale sclerose ziekte van Parkinson α-synuclein gesmolten in Sarcoom TAR DNA-bindende eiwit 43 histone posttranslationele modificaties epigenetica
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