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JoVE Journal Genetics
Karakterisere Histone post-translasjonell modifikasjon endringer i gjær nevrodegenerative Proteinopathy modeller
Surveying Low-Cost Methods to Measure Lifespan and Healthspan in Caenorhabditis elegans
Journal (Genetics)
Surveying Low-Cost Methods to Measure Lifespan and Healthspan in Caenorhabditis elegans
RNAi Inheritance and ChIP in C. elegans (Video) | JoVE
Journal (Genetics)
RNAi Inheritance and ChIP in C. elegans (Video) | JoVE
 
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Karakterisere Histone post-translasjonell modifikasjon endringer i gjær nevrodegenerative Proteinopathy modeller

Article DOI: 10.3791/59104-v • 08:33 min • March 24th, 2019
March 24th, 2019 •
1,2, 1,3, 1, 1, 1, 1, 1,4
1Chemistry Department, Brooklyn College, 2Ph.D. Program in Biochemistry, Graduate Center of the City University of New York, 3Ph.D. Program in Chemistry, Graduate Center of the City University of New York, 4Ph.D. Programs in Chemistry, Biochemistry, and Biology, Graduate Center of the City University of New York

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Denne protokollen skisserer eksperimentelle prosedyrer for å karakterisere genomet-omfattende endringer i nivåer av histone post-translasjonell endringer (PTM) oppstår i forbindelse med overuttrykte proteiner knyttet ALS og Parkinsons Saccharomyces cerevisiae modeller. Etter SDS side separasjon oppdages personlige histone PTM nivåer med endring-spesifikke antistoffer via Western blotting.

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Genetikk problemet 145 neurodegeneration amyotrofisk lateral sklerose Parkinsons sykdom α-synuclein smeltet i sarkomspesialitet TAR DNA-bindende protein 43 histone post-translasjonell modifikasjoner epigenetics
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