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Journal / Genetics / 慢病毒载体平台,…
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Genetics

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JoVE Journal Genetics
慢病毒载体平台, 用于将表观基因编辑工具高效地传递到人类诱导的多能干细胞衍生疾病模型中
In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
Journal (Genetics)
In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
Detection of Copy Number Alterations Using Single Cell Sequencing
Journal (Genetics)
Detection of Copy Number Alterations Using Single Cell Sequencing
 
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慢病毒载体平台, 用于将表观基因编辑工具高效地传递到人类诱导的多能干细胞衍生疾病模型中

Article DOI: 10.3791/59241-v • 13:47 min • March 29th, 2019
March 29th, 2019 •
1,2, 3, 3, 1, 1,2, 4, 4, 1,2, 3
1Department of Neurology, Duke University Medical Center, 2Center for Genomic and Computational Biology, Duke University Medical Center, 3Viral Vector Core, Duke University Medical Center, 4Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Duke University Medical Center

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靶向 DNA 表观基因组编辑是一种强有力的治疗方法。该协议描述了一种一体式慢病毒载体的生产、纯化和浓度, 这些载体窝藏 CRISPR9-DNMT3A 转导基因, 用于人类诱导的多能干细胞 (hiPSC) 衍生神经元的表观基因编辑应用。

Tags

遗传学 第145期 慢病毒载体 dnmt3a Crispr-dcas9 SNCA Hpsc 甲基化 焦磷酸测序 表观基因组编辑 帕金森病
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