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Biochemistry

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JoVE Journal Biochemistry
Uttrykk og rensing av Nuklease-gratis Oxygen renovasjons Protocatechuate 3, 4-Dioxygenase
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Uttrykk og rensing av Nuklease-gratis Oxygen renovasjons Protocatechuate 3, 4-Dioxygenase

Article DOI: 10.3791/59599-v • 10:14 min • November 8th, 2019
November 8th, 2019 •
1, 1, 1, 1
1Cancer Biology and Genetics, College of Medicine, The Ohio State University

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Protocatechuate 3, 4-dioxygenase (PCD) kan enzymatisk fjerne fritt diatomic oksygen fra et vandig system ved hjelp av sitt substrat protocatechuic syre (PCA). Denne protokollen beskriver uttrykket, rensing, og aktivitet analyse av dette oksygen rensing enzymet.

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Biokjemi kromatografi protocatechuate-3 4-dioxygenase (PCD) nuklease forurensning protocatechuic acid (PCA) reaktive oksygen arter oksygen rensing systemer (OSS)
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