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Biochemistry

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JoVE Journal Biochemistry
Utvärdering av protein-proteininteraktioner med hjälp av en on-membran rötnings teknik
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Utvärdering av protein-proteininteraktioner med hjälp av en on-membran rötnings teknik

Article DOI: 10.3791/59733-v • 07:07 min • July 19th, 2019
July 19th, 2019 •
*1, *2, 1, 2, 1
1Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, 2Department of Biochemistry, Showa University School of Medicine
* These authors contributed equally

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Här presenterar vi ett protokoll för en membran matsmältning teknik för beredning av prover för masspektrometri. Denna teknik underlättar den praktiska analysen av protein-proteininteraktioner.

Tags

Biokemi immunoprecipitation LC/MS/MS proteomik protein interaktion PVDF-membran screening
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