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Journal / Immunology and Infection / Un test di tubercolosi a carico batterico…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Un test di tubercolosi a carico batterico molecolare (TB-MBLA)
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Un test di tubercolosi a carico batterico molecolare (TB-MBLA)

Article DOI: 10.3791/60460-v • 10:41 min • April 30th, 2020
April 30th, 2020 •
1, 1,2, 1, 1, 1, 3, 4, 1, 1
1Division of Infection and Global Health, School of Medicine, University of St Andrews, 2National Institute for Medical Research (NIMR)-Mbeya Medical Research Centre, 3Instituto Nacional de Saúde (INS), Ministério da Saúde, 4Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medical, Veterinary & Life Sciences, University of Glasgow

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Descriviamo un test di analisi del carico batterico molecolare della tubercolosi eseguito dopo l'inattivazione termica dell'espettorato. L'inattivazione del calore rende non infettivi i campioni di sputum e ovvia la necessità di laboratori di livello di contenimento 3 per i test molecolari della tubercolosi.

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Immunologia e Infezione Numero 158 tubercolosi Mycobacterium tuberculosis viabilità inattivazione del calore RNA analisi del carico batterico molecolare trascrizione inversa PCR quantitativa
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