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Journal / Genetics / 3D Multicolor DNA FISH Tool to Study Nuclear…
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Genetics

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JoVE Journal Genetics
3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells 3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells 3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells 3D
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3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells 3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells 3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells 3D

Article DOI: 10.3791/60712-v • 11:25 min • January 25th, 2020
January 25th, 2020 •
1, 1, 1, 1
1Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi", INGM

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3D multicolor DNA FISH representerar ett verktyg för att visualisera flera genomiska loci inom 3D bevarade kärnor, entydigt definiera deras ömsesidiga interaktioner och lokalisering inom kärnområdet på en enda cell nivå. Här beskrivs ett steg för steg protokoll för ett brett spektrum av mänskliga primära celler.

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Genetik fluorescens in situ hybridisering 3D multicolor DNA FISH konserverad ephase kärnor nukleär arkitektur 3D-genom vikning DNA-kontakter nukleär positionering mänskliga primära celler
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