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Bioengineering

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JoVE Journal Bioengineering
Forberedelse proteinproducerende syntetiske celler ved hjælp af cellefri bakterielle ekstrakter, liposomer og emulsionoverførsel
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Forberedelse proteinproducerende syntetiske celler ved hjælp af cellefri bakterielle ekstrakter, liposomer og emulsionoverførsel

Article DOI: 10.3791/60829-v • 09:37 min • April 27th, 2020
April 27th, 2020 •
*1,2, *1,2, *1,3, 1, 1,3, 1, 1, 1
1Laboratory for Targeted Drug Delivery and Personalized Medicine Technologies, Department of Chemical Engineering, Technion - Israel Institute of Technology, 2The Norman Seiden Multidisciplinary Program for Nanoscience and Nanotechnology, Technion - Israel Institute of Technology, 3The Interdisciplinary Program for Biotechnology, Technion - Israel Institute of Technology
* These authors contributed equally

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Denne protokol beskriver metode, materialer, udstyr og trin til bottom-up forberedelse af RNA og proteinproducerende syntetiske celler. Den indre vandige rum af de syntetiske celler indeholdt S30 bakterielle lysat indkapslet i en lipid tolags (dvs. stabile liposomer), ved hjælp af en vand-i-olie emulsion overførsel metode.

Tags

Bioengineering Syntetiske celler cellefri emulsion bakteriel lysat kunstige celler lægemiddellevering terapeutiske proteiner GUI'er liposom proteocell protocell proteoliposome biologi rettet evolution syntetisk biologi
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