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Brug caenorhabditis elegans til skærmen for vævsspecifikke chaperone interaktioner

Article DOI: 10.3791/61140-v • 06:55 min • June 7th, 2020
June 7th, 2020 •
1, 1, 1, 1
1Department of Life Sciences, Ben-Gurion University of the Negev

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For at studere chaperone-chaperone og chaperone-substrat interaktioner, udfører vi syntetiske interaktion skærme i Caenorhabditis elegans ved hjælp af RNA interferens i kombination med milde mutationer eller over-udtryk for chaperoner og overvåge vævsspecifikke protein dysfunktion på organismeniveau.

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Biologi Caenorhabditis elegans chaperone genetiske interaktioner proteostase RNAi skærm temperaturfølsomme
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