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Journal / Immunology and Infection / T- und B-Zell-Rezeptor-Immunrepertoireanalyse…
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Immunology and Infection

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JoVE Journal Immunology and Infection
T- und B-Zell-Rezeptor-Immunrepertoireanalyse mittels Next-Generation-Sequencing
Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
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Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions
Journal (Immunology and Infection)
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T- und B-Zell-Rezeptor-Immunrepertoireanalyse mittels Next-Generation-Sequencing

Article DOI: 10.3791/61792-v • 08:59 min • January 12th, 2021
January 12th, 2021 •
1,2, 3,4, 1, 3,4, 1,2
1Pediatric Gastroenterology Unit, Edmond and Lily Safra Children's Hospital, Sheba Medical Center, 2Sackler Faculty of Medicine, Tel Aviv University, 3Cancer Research Center, Sheba Medical Center, 4Molecular Hematology Laboratory, Sheba Medical Center

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Das aktuelle Protokoll beschreibt eine Methode zur DNA-Isolierung aus Blutproben und Darmbiopsien, die Generierung von TCRβ- und IGH-PCR-Bibliotheken für die Sequenzierung der nächsten Generation, die Durchführung eines NGS-Laufs und die grundlegende Datenanalyse.

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Next-generation sequencing TCRβ IGH Immunerepertoire adaptive Immunität T-Zellen B-Zellen
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