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Kvantitativ Proteomics Workflow ved hjælp af flere reaktionsovervågningsbaserede detektioner af proteiner fra humant hjernevæv

Article DOI: 10.3791/61833-v • 11:49 min • August 28th, 2021
August 28th, 2021 •
*1, *2, 2
1Centre for Research in Nanotechnology and Science, Indian Institute of Technology Bombay, 2Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay
* These authors contributed equally

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Protokollen har til formål at indføre brugen af et tredobbelt firdobbelt massespektrometer til multireaktionsovervågning (MRM) af proteiner fra kliniske prøver. Vi har leveret en systematisk arbejdsgang fra prøveforberedelse til dataanalyse for kliniske prøver med alle de nødvendige forholdsregler, der skal træffes.

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