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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualización Del SARS-CoV-2 Usando Inmuno ARN-Fluorescencia Hibridación In Situ
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Visualización Del SARS-CoV-2 Usando Inmuno ARN-Fluorescencia Hibridación In Situ

Article DOI: 10.3791/62067-v • 05:23 min • December 23rd, 2020
December 23rd, 2020 •
1, 2, 1, 1,2, 1,2, 3,4, 3,4, 1
1Malopolska Centre of Biotechnology, Jagiellonian University, 2Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 3Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, The Medical University of Silesia in Katowice, 4Silesian Centre for Heart Diseases

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Aquí, describimos un método simple que combina el hibridación in situ de la fluorescencia del ARN (RNA-PESCADO) con la inmunofluorescencia para visualizar el ARN agudo severo del coronavirus 2 del coronavirus del síndrome respiratorio (SARS-CoV-2). Este protocolo puede aumentar la comprensión de las características moleculares de las interacciones ARN-huésped SARS-CoV-2 a nivel unicelular.

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Inmunología e Infección Número 166 SARS-CoV-2 ARN-FISH Reacción en cadena de hibridación Inmunofluorescencia Epitelio de las Vías Respiratorias Humanas (AVA) Permeabilización Microscopía Confocal
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