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Journal / Behavior / CRISPR/Cas9ゲノム編集 ヘリコベルパ・アルミゲラ…
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JoVE Journal Behavior
CRISPR/Cas9ゲノム編集 ヘリコベルパ・アルミゲラ (Hübner)の胚マイクロインジェクションとノックアウト変異体の同定
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CRISPR/Cas9ゲノム編集 ヘリコベルパ・アルミゲラ (Hübner)の胚マイクロインジェクションとノックアウト変異体の同定

Article DOI: 10.3791/62068-v • 06:37 min • July 1st, 2021
July 1st, 2021 •
1,2, 2, 1, 1, 1, 2,3
1School of Chemical and Environmental Engineering, China University of Mining and Technology, 2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 3Guangdong Laboratory of Lingnan Modern Agriculture, Shenzhen; Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences

章节

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ここでは、CRISPR/Cas9ゲノム編集によって作成された Helicoverpa armigera (Hübner)胚マイクロインジェクションとノックアウト変異体同定のプロトコルを紹介します。変異昆虫は、生体内の異なる遺伝子間の遺伝子機能および相互作用のさらなる研究を可能にする。

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行動 Helicoverpa armigera 胚マイクロインジェクション 遺伝子ノックアウト CRISPR Cas9
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