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Immunology and Infection

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JoVE Journal Immunology and Infection
Analisi citometrica a flusso per l'identificazione delle cellule immunitarie innate e adattative del polmone murino
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Analisi citometrica a flusso per l'identificazione delle cellule immunitarie innate e adattative del polmone murino

Article DOI: 10.3791/62985-v • 09:57 min • November 16th, 2021
November 16th, 2021 •
1,2, 1,2,3, 1,2, 1,2, 1,2
1Division of Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 2Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 3Harvard College

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In questo studio, presentiamo un protocollo efficace e riproducibile per isolare le popolazioni immunitarie del sistema respiratorio murino. Forniamo anche un metodo per l'identificazione di tutte le cellule immunitarie innate e adattative che risiedono nei polmoni di topi sani, utilizzando un pannello di citometria a flusso a 9 colori.

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Immunologia e infezione Numero 177 polmone cellule immunitarie strategia di gating citometria a flusso
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