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JoVE Journal Genetics
Indel-Nachweis nach CRISPR/Cas9-Mutagenese mittels hochauflösender Schmelzeanalyse in der Mücke Aedes aegypti
Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
Journal (Genetics)
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Indel-Nachweis nach CRISPR/Cas9-Mutagenese mittels hochauflösender Schmelzeanalyse in der Mücke Aedes aegypti

Article DOI: 10.3791/63008-v • 05:30 min • September 10th, 2021
September 10th, 2021 •
1, 1, 1, 1, 1
1Department of Entomology and Agrilife Research, Texas A&M University

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Dieser Artikel beschreibt ein Protokoll zur schnellen Identifizierung von Indeln, die durch CRISPR/Cas9 induziert werden, und zur Auswahl von Mutantenlinien in der Mücke Aedes aegypti unter Verwendung einer hochauflösenden Schmelzanalyse.

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Genetik Heft 175
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