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Het opzetten van menselijke longorganoïden en proximale differentiatie om volwassen luchtwegorganoïden te genereren

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Het opzetten van menselijke longorganoïden en proximale differentiatie om volwassen luchtwegorganoïden te genereren

Article DOI: 10.3791/63684-v • 10:12 min • March 23rd, 2022

March 23rd, 2022 •

Cun Li^1,2, Man Chun Chiu^1,2, Yifei Yu^1,2, Xiaojuan Liu^1,2, Ding Xiao^1,2, Jingjing Huang^1,2, Zhixin Wan^1,2, Jie Zhou^1,2,3

¹Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, ²Centre for Virology, Vaccinology and Therapeutics, Hong Kong Science and Technology Park, ³State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong

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Het protocol presenteert een methode om menselijke longorganoïden af te leiden uit primaire longweefsels, de longorganoïden uit te breiden en proximale differentiatie te induceren om 3D- en 2D-luchtwegorganoïden te genereren die het menselijke luchtwegepitheel getrouw fenokopie.

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Biologie Nummer 181

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