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Retinal Explant of the Adult Mouse Retina as an Ex Vivo Model for Studying Retinal Neurovascular Diseases

Article DOI: 10.3791/63966-v • 06:51 min • December 9th, 2022
December 9th, 2022 •
1,2,3, 1,2, 1,2
1Eye Research Center and Department of Foundational Medical Studies, Oakland University William Beaumont School of Medicine, 2Eye Research Institute, Oakland University, 3Human Anatomy and Embryology Department, Mansoura Faculty of Medicine, Mansoura University

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This protocol presents and describes steps for the isolation, dissection, culturing, and staining of retinal explants obtained from an adult mouse. This method is beneficial as an ex vivo model for studying different retinal neurovascular diseases such as diabetic retinopathy.

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Retinal Explant Adult Mouse Retina Ex Vivo Model Retinal Neurovascular Diseases Retinal Research Crosstalk Between Cells Retinal Neurons Glial Cells Vascular Cells Cultured Retinal Explant Biochemical Parameters Vascular System Screening Tool Pharmacological Interventions Retinovascular Diseases Neurodegenerative Diseases Diabetic Retinopathy Vascular And Neuronal Injury Pathophysiological Changes Microvascular Retinal Dysfunction In Vivo Models In Vitro Models
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