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JoVE Journal Genetics
Udvikling af knock-out muskelcellelinjer ved hjælp af Lentivirus-medieret CRISPR / Cas9 genredigering
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
Journal (Genetics)
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation
Journal (Genetics)
Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation
 
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Udvikling af knock-out muskelcellelinjer ved hjælp af Lentivirus-medieret CRISPR / Cas9 genredigering

Article DOI: 10.3791/64114-v • 10:12 min • June 16th, 2022
June 16th, 2022 •
1, 1, 1, 2, 2, 1, 1
1University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, 2Genome Center, University of California, Davis

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Protokollen beskriver, hvordan man genererer knock-out myoblaster ved hjælp af CRISPR/Cas9, startende fra design af guide-RNA'er til den cellulære kloning og karakterisering af knock-out klonerne.

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Genetik udgave 184
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