Tianjin Medical University General Hospital 4 articles published in JoVE Neuroscience Establishment of Central Cord Syndrome Model in C57BL/6J Mouse Elham Yilizati-Yilihamu Elzat*1, Xiangchuang Fan*1, Shiqing Feng1,2 1Department of Orthopaedics, Qilu Hospital of Shandong University, 2Department of Orthopaedics, Tianjin Medical University General Hospital The present protocol simulating central cord syndrome (CCS) in mice has improved repeatability and minimized operation damage to the experimental animals, avoiding disrupting the anatomical structure excessively. The strategy in this study is advantageous because it allows for research into injury mechanisms by producing consistent results. Medicine Determination of the Procoagulant Activity of Extracellular Vesicle (EV) Using EV-Activated Clotting Time (EV-ACT) Yalong Gao1,2,3, Kaiji Li1,2, Qiong Qin4, Jianning Zhang1,2, Li Liu1 1Key Laboratory of Post-Neurotrauma Neurorepair and Regeneration in Central Nervous System, Ministry of Education and Tianjin Neurological Institute, Tianjin Medical University General Hospital, 2Department of Neurosurgery, Tianjin Medical University General Hospital, 3Department of Neurosurgery, Tianjin Huanhu Hospital, 4Department of Medical Oncology, Tianjin Medical University General Hospital This protocol investigates the use of extracellular vesicle (EV)-rich plasma as an indicator of the coagulative ability of EV. EV-rich plasma is obtained through a process of differential centrifugation and subsequent recalcification. Neuroscience Establishing a Mouse Contusion Spinal Cord Injury Model Based on a Minimally Invasive Technique Elham Yilizati-Yilihamu Elzat1, Xiangchuang Fan1, Zimeng Yang1, Zhongze Yuan1, Yilin Pang2, Shiqing Feng1,2 1Department of Orthopaedics, Qilu Hospital of Shandong University, 2Department of Orthopaedics, Tianjin Medical University General Hospital Minimally invasive techniques and a simple laboratory device improve the reproducibility of the spinal cord injury model by reducing operative damage to the experimental animals and allowing anatomical morphology maintenance. The method is worthwhile because the reliable results and reproducible procedure facilitate investigations of the mechanisms of disease reparation. Medicine International Expert Consensus and Recommendations for Neonatal Pneumothorax Ultrasound Diagnosis and Ultrasound-guided Thoracentesis Procedure Jing Liu1,2, Dalibor Kurepa3, Francesco Feletti4,5, Almudena Alonso-Ojembarrena6, Jovan Lovrenski7, Roberto Copetti8, Erich Sorantin9, Javier Rodriguez-Fanjul10, Karishma Katti3, Andrea Aliverti4, Huayan Zhang11,12, Misun Hwang13, Tsu F. Yeh14, Cai-Bao Hu15, Xing Feng16, Ru-Xin Qiu1,2, Jing-Han Chi17, Li-Li Shang18, Guo-Rong Lyu19, Shao-Zheng He20, Yan-Fen Chai21, Zhan-Jun Qiu22, Hai-Ying Cao2,23, Yue-Qiao Gao1,2, Xiao-Ling Ren1,2, Guo Guo1,24, Li Zhang1,2, Ying Liu1,2, Wei Fu1,2, Zu-Lin Lu1,2, Hong-Lei Li1,2 1Department of Neonatology and NICU, Beijing Chaoyang District Maternal and Child Healthcare Hospital, 2The National Neonatal Lung Ultrasound Training Base, 3 Pneumothorax is a common emergency and critical disease in newborn infants that needs rapid, clear diagnosis and timely treatment. Diagnosis and treatment based on chest X-rays are associated with delayed management and radiation damage. Lung ultrasound (US) provides useful guidance for rapid, accurate diagnosis and the precise thoracentesis of pneumothorax.
