Indian Institute of Technology Ropar 1 article published in JoVE Cancer Research Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts Isolated from 3D Multicellular Lung Tumor Spheroids Leena Arora*1, Moyna Kalia*1, Soumyajit Roy1, Durba Pal1 1Department of Biomedical Engineering, Indian Institute of Technology Ropar Multicellular 3D tumor spheroids were prepared with lung adenocarcinoma cells, fibroblasts, and monocytes, followed by the isolation of cancer-associated fibroblasts (CAFs) from these spheroids. Isolated CAFs were compared with normal fibroblasts to assess mitochondrial health by studying the mitochondrial transmembrane potential, reactive oxygen species, and enzymatic activities.
