Institucio Catalana de Recerca i Estudis Avancats (ICREA) 4 articles published in JoVE Medicine Non-Invasive Monitoring of Microvascular Oxygenation and Reactive Hyperemia using Hybrid, Near-Infrared Diffuse Optical Spectroscopy for Critical Care M. Atif Yaqub*1, Marta Zanoletti*1, Lorenzo Cortese1, Daniel Senciales Sánchez1, Caterina Amendola2, Lorenzo Frabasile2, Umut Karadeniz1, Jacqueline Martinez Garcia1, Marta Martin1, Jordi Cortes-Picas1, Alba Caballer3, Edgar Cortes3, Sara Nogales3, Alberto Tosi4, Talyta Carteano5, Diego Sanoja Garcia5, Jakub Tomanik6, Tessa Wagenaar6, Hsiao Mui6, Claudia Nunzia Guadagno7, Shahrzad Parsa8, Sanathana Konugolu Venkata Sekar7, Luc Demarteau6, Tijl Houtbeckers6, Udo M. Weigel8, Michele Lacerenza9, Mauro Buttafava9, Alessandro Torricelli2,10, Davide Contini2, Jaume Mesquida3, Turgut Durduran1,11 1ICFO - Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, 2Dipartimento di Fisica, Politecnico di Milano, 3Critical Care Department, Parc Taulí Hospital Universitari, 4Dipartimento di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, 5ASPHALION s.l., 6Splendo, 7BioPixS-Biophotonics Standards, IPIC, Tyndall National Institute, 8Hemophotonics s.l., 9PIONIRS s.r.l., 10Consiglio Nazionale delle Ricerche, Istituto di Fotonica e Nanotecnologie, 11Institució Catalana de Recerca i Estudis Avançats (ICREA) We describe a protocol to non-invasively and continuously measure absolute microvascular blood flow index and blood oxygen saturation using a multi-modal device based on near-infrared diffuse optics. We then evaluate the metabolic rate of oxygen consumption and reactive hyperemia utilizing a vascular occlusion test. Neuroscience Calcium Imaging In Electrically Stimulated Flat-Mounted Retinas Marina Cunquero1, Maria Marsal1, Gustavo Castro-Olvera1, Steven T. Walston2, F. Taygun Duvan2, José Gabriel Macias-Montero3, Carles Puigdengoles3, Mokhtar Chmeissani3, Jose A. Garrido2,4, Pablo Loza-Alvarez1 1Institut de Ciències Fotòniques (ICFO), The Barcelona Institute of Science and Technology, 2Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Campus UAB, 3Institut de Física d'Altes Energies (IFAE), The Barcelona Institute of Science and Technology, Campus UAB, 4Institució Catalana de Recerca i Estudis Avançats (ICREA) Retinal prostheses have the ability to generate visual perceptions. To advance the development of new prostheses, ex vivo methods are needed to test devices before implantation. This article provides a comprehensive protocol for studying calcium activity in the retinal ganglion cell layer when subjected to electrical stimulation. Engineering Glass-Based Devices to Generate Drops and Emulsions Josefa Guerrero1, Javier Rojo2, Alexis de la Cotte2, Luis Manuel Aguilera-Sáez3, Enric Vila3, Alberto Fernandez-Nieves2,4,5 1Department of Chemistry and Physics, Augusta University, 2Department of Condensed Matter Physics, University of Barcelona, 3Agrobío S.L., 4ICREA - Institució Catalana de Recerca i Estudis Avançats, 5Institute of Complex Systems (UBICS), University of Barcelona Here, a protocol to manufacture glass-based microfluidic devices used for generating highly monodisperse emulsions with controlled drop size is presented. Bioengineering Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation Ignasi Casanellas1,2, Anna Lagunas3,1, Iro Tsintzou1, Yolanda Vida4,5, Daniel Collado4,5, Ezequiel Pérez-Inestrosa4,5, Cristina Rodríguez-Pereira6, Joana Magalhaes3,6, Pau Gorostiza1,3,7, José A. Andrades8,3, José Becerra8,3,5, Josep Samitier1,3,2 1Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 2Department of Engineering Electronics, University of Barcelona (UB), 3Networking Biomedical Research Center (CIBER), 4Instituto de Investigacin Biomédica de Málaga (IBIMA), Department of Organic Chemistry, Universidad de Málaga (UMA), 5Andalusian Centre for Nanomedicine and Biotechnology-BIONAND, 6Unidad de Bioingeniería Tisular y Terapia Celular (GBTTC-CHUAC), Grupo de Reumatolog ía, Instituto de Investigación Biomèdica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas, Universidade da Coruña (UDC), 7Institució Catalana de Recerca i Estudis Avançats (ICREA), 8Instituto de Investigación Biomédica de Málaga (IBIMA), Department of Cell Biology, Genetics and Physiology, Universidad de Málaga (UMA) A method to obtain dendrimer-based uneven nanopatterns that permit the nanoscale control of local arginine-glycine-aspartic acid (RGD) surface density is described and applied for the study of cell adhesion and chondrogenic differentiation.
