University of Jaen 1 article published in JoVE Developmental Biology Live Imaging of Early Cardiac Progenitors in the Mouse Embryo Miquel Sendra1, Jorge Mañes1, Jorge N. Domínguez2,3, Miguel Torres1 1Cardiovascular Regeneration Program, Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Cardiovascular Development Group, Department of Experimental Biology, University of Jaén, Jaén, 3Fundación Medina, Granada We present a detailed protocol for mouse embryo culture and imaging that enables 3D + time imaging of cardiac progenitor cells. This video-toolkit addresses the key skills required for successful live imaging otherwise hard to acquire from text-only publications.