Italian Cystic Fibrosis Research Foundation 1 article published in JoVE Immunology and Infection Long Term Chronic Pseudomonas aeruginosa Airway Infection in Mice Marcella Facchini1,2, Ida De Fino1,2, Camilla Riva1,2, Alessandra Bragonzi1 1Infections and Cystic Fibrosis Unit, Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, 2Italian Cystic Fibrosis Research Foundation We describe the agar-beads method to establish persistent long-term chronic Pseudomonas aeruginosa airway infection in the mouse model.