William Beaumont School of Medicine 1 article published in JoVE Biology Isolation of Primary Mouse Retinal Pigmented Epithelium Cells Ryan Tomaszewski1,2, Pragya Rajpurohit3, Mei Cheng1,2, Amany Tawfik1,2,3 1Eye Research Institute, Oakland University, 2Eye Research Center (OUWB)/ERC, William Beaumont School of Medicine, 3Oral Biology and Diagnostic Sciences, Dental College of Georgia, Augusta University This manuscript describes a simplified protocol for the isolation of retinal pigmented epithelium (RPE) cells from mouse eyes in a stepwise manner. The protocol includes the enucleation and dissection of mouse eyes, followed by the isolation, seeding, and culturing of RPE cells.