Instituto Nacional de Tecnologia 1 article published in JoVE Biochemistry Preparation of Hard Palm Seeds for Matrix-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry Analysis Gabriel R. Martins*1,2, Felipe L. Brum*1,2,3, Davi Marconi Miranda Carvalho da Silva*1,2, Livia C. Barbosa3, Ronaldo Mohana-Borges3, Ayla Sant'Ana da Silva1,2 1Laboratório de Biocatálise (LABIC), Instituto Nacional de Tecnologia, 2Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, 3Centro de Espectrometria de Massas de Biomoléculas, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro This protocol aimed to describe detailed guidance on the preparation of hard seed sample sections with low water content for MALDI-IMS analysis, maintaining analytes' original distribution and abundance and providing high-quality signal and spatial resolution.
