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Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Article DOI: 10.3791/1325-v • 19:16 min • August 5th, 2009
August 5th, 2009 •
1, 1
1Department of Cell Biology, Scripps Institute

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Summary
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Selection, microinjection, and imaging of fluorescently-labeled F-actin via fluorescent speckle microscopy (FSM).

Tags

Fluorescent Speckle Microscopy F-actin Dynamics Live Cell Imaging FSM Actin Dynamics PtK1 Cells Turnover Kinetics Assembly/disassembly Computer Vision Software QFSM Fluorescent Actin Probes Macromolecular Assemblies Microtubules Intermediate Filaments Adhesion Complexes
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