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Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
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Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

Article DOI: 10.3791/50521-v • 07:50 min • July 23rd, 2013
July 23rd, 2013 •
*1, *1, 1, 1, 1, 1, 1
1The College of Technology and Innovation, Center for Infectious Disease and Vaccinology, The Biodesign Institute, Arizona State University
* These authors contributed equally

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Summary
Automatic Translation

Plants offer a novel system for the production of pharmaceutical proteins on a commercial scale that is more scalable, cost-efficient and safe than current expression paradigms. In this study, we report a simple and convenient, yet scalable approach to introduce target-gene containing Agrobacterium tumefaciens into plants for protein transient expression.

Tags

Agroinfiltration Plants Transient Expression Recombinant Proteins Mammalian Cell Culture Pharmaceutical Production Virus-based Vectors Target-gene Agrobacterium Leaves Fluorescent Proteins GFP DsRed Syringe Infiltration Vacuum Infiltration Laboratory Scale Expression
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