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JoVE Journal Developmental Biology
Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line
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Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line

Article DOI: 10.3791/53193-v • 08:07 min • February 17th, 2016
February 17th, 2016 •
1, 1, 1
1Department of Biochemistry and Molecular Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University

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Summary
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It is critical in neurobiology and neurovirology to have a reliable, replicable in vitro system that serves as a translational model for what occurs in vivo in human neurons. This protocol describes how to culture and differentiate SH-SY5Y human neuroblastoma cells into viable neurons for use in in vitro applications.

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SH-SY5Y Human Neuroblastoma Cell Line Differentiation Protocol Neurobiologists Neurovirologists In Vitro Assays Infectious Disease Research Neuronal Responses Drug Treatment Proteomic Changes Genomic Changes Culture Of Differentiated Human Neurons Reproducible Method Homogenous Cultures Sensitivity Of Cells Trypsinization Triteration Environmental Conditions T-75 Flask PBS Trypsin EDTA
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