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Journal / Biochemistry / Spektrofotometri fastsettelse av Phycobiliprotein …
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Biochemistry
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JoVE Journal Biochemistry
Spektrofotometri fastsettelse av Phycobiliprotein innhold i Cyanobacterium Synechocystis
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Spektrofotometri fastsettelse av Phycobiliprotein innhold i Cyanobacterium Synechocystis

Article DOI: 10.3791/58076-v • 08:08 min • September 11th, 2018
September 11th, 2018 •
1, 1,2, 3, 1
1Department of Adaptive Biotechnologies, Global Change Research Institute, Czech Academy of Sciences, 2Department of Plant Physiology, Faculty of Science, Masaryk University, 3Laboratory of Intracellular Regulation, Institute of Plant Physiology, Russian Academy of Sciences

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Her presenterer vi en protokoll for å finne kvantitativt phycobiliprotein innhold i cyanobacterium Synechocystis med en Spektrofotometri metode. Utvinning prosedyren ble også brukt til andre Cyanobakterie og alger stammer; men på grunn av variasjoner i pigment absorpsjon spectra er det nødvendig å teste hver stamme Spektrofotometri ligninger individuelt.

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Biokjemi problemet 139 Phycobilisomes phycobilins phycocyanobilins phycocyanin allophycocyanin pigmenter lys høsting protein Cyanobakterie alger spectrophotometry utvinning
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