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Immunology and Infection

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Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells

Article DOI: 10.3791/62984-v • 06:55 min • October 19th, 2021
October 19th, 2021 •
*1, *1, 1, 1, 1,3, 2, 1
1National Center for Cancer Immune Therapy, Department of Oncology, University Hospital Herlev, 2Department of Cellular and Molecular Medicine, Center for Healthy Aging, University of Copenhagen, 3Department of Immunology and Microbiology, Inflammation and Cancer Group, University of Copenhagen
* These authors contributed equally

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Summary
Automatic Translation

Metabolic adaptation is fundamental for T cells as it dictates differentiation, persistence, and cytotoxicity. Here, an optimized protocol for monitoring mitochondrial respiration in ex vivo cytokine-differentiated human primary T cells is presented.

Tags

Real-time Monitoring Mitochondrial Respiration Cytokine-differentiated Human Primary T Cells Seahorse Machine Live Cell Analysis T Cell Durability T Cell Memory Labeling-free Measurement T Cell Fitness Memory Potential Cancer Immunotherapy CD3/CD28 Beads Wash Beads PBS Buffer Resuspend Beads T Cell Medium Cytokine Treatment Multi-well Plates Incubation
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