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Journal / Biochemistry / En robust kryo-elektronmikroskopi (cryo-EM)…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
En robust kryo-elektronmikroskopi (cryo-EM) prosesseringsarbeidsflyt med kryoSPARC, RELION og scipion
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En robust kryo-elektronmikroskopi (cryo-EM) prosesseringsarbeidsflyt med kryoSPARC, RELION og scipion

Article DOI: 10.3791/63387-v • 13:43 min • January 31st, 2022
January 31st, 2022 •
1, 1,2
1Institute for Quantitative Biomedicine, Rutgers University, 2Department of Biochemistry & Microbiology, Rutgers University

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Denne artikkelen beskriver hvordan du effektivt bruker tre cryo-EM-prosesseringsplattformer, det vil si cryoSPARC v3, RELION-3 og Scipion 3, for å lage en enkelt og robust arbeidsflyt som gjelder for en rekke enkeltpartikkeldatasett for høyoppløselig strukturbestemmelse.

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Biokjemi Utgave 179 kryo-elektronmikroskopi kryo-EM enkeltpartikkelanalyse SPA kryoSPARC RELION Scipion bildebehandling strukturberegning AAV
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