Wuhan Center for Disease Control and Prevention 1 article published in JoVE Neuroscience Neuronal Differentiation from Mouse Embryonic Stem Cells In vitro Xiang Mao*1, Shasha Zhao*2 1Wuhan Center for Disease Control and Prevention, 2College of Life Science and Health, Wuhan University of Science and Technology Here, we established a low cost and easy to operate method that directs fast and efficient differentiation from embryonic stem cells into neurons. This method is suitable for popularization among laboratories and can be a useful tool for neurological research.