Osaka City University 1 article published in JoVE Biology Methods for Staging Pupal Periods and Measurement of Wing Pigmentation of Drosophila guttifera Yuichi Fukutomi1, Keiji Matsumoto1,2, Noriko Funayama1, Shigeyuki Koshikawa1,3,4 1Graduate School of Science, Kyoto University, 2Graduate School of Science, Osaka City University, 3The Hakubi Center for Advanced Research, Kyoto University, 4Graduate School of Environmental Science, Hokkaido University Protocols for staging pupal periods and measurement of wing pigmentation of Drosophila guttifera are described. Staging and quantification of pigmentation provide a solid basis for studying developmental mechanisms of adult traits and enable interspecific comparison of trait development.