Kyung Hee University 7 articles published in JoVE Biology Isolation of Epithelial Cells from Human Dental Follicle Hyosun Jung1, Jin-Kyu Yi1,2 1Department of Conservative Dentistry, Kyung Hee University Dental Hospital at Gangdong, 2Department of Conservative Dentistry, School of Dentistry, Kyung Hee University The dental follicle contains an epithelial population and mesenchymal cells. The epithelial population was selected from the heterogeneous dental follicle cell population by providing a distinct culture medium. Epithelial cells survived and formed colonies in a serum-free medium. Medicine Electrocardiogram Recordings in Anesthetized Mice using Lead II Tae Woong Ha1, Bermseok Oh*1, Ji-One Kang*1 1Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University We present an ECG protocol that is technically easy, inexpensive, fast, and affordable in small mice, and can be performed with enhanced sensitivity. We suggest this method as a screening approach for studying pharmacological agents, genetic modifications, and disease models in mice. Environment A Novel Method for the Pentosan Analysis Present in Jute Biomass and Its Conversion into Sugar Monomers Using Acidic Ionic Liquid Babasaheb M. Matsagar1, Shahriar A. Hossain2,3, Tofazzal Islam4, Yusuke Yamauchi2,3,5,6, Kevin C.-W. Wu1 1Department of Chemical Engineering, National Taiwan University, 2International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 3Australian Institute for Innovative Materials (AIIM), University of Wollongong, 4Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, 5School of Chemical Engineering & Australian Institute for Bioengineering and Nanotechnology (AIBN), University of Queensland, 6Department of Plant and Environmental New Resources, Kyung Hee University We present a protocol for the synthesis of C5 sugars (xylose and arabinose) from a renewable non-edible lignocellulosic biomass (i.e., jute) with the presence of Brønsted acidic ionic liquids (BAILs) as the catalyst in water. The BAILs catalyst exhibited better catalytic performance than conventional mineral acid catalysts (H2SO4 and HCl). Chemistry Chemical Synthesis of Porous Barium Titanate Thin Film and Thermal Stabilization of Ferroelectric Phase by Porosity-Induced Strain Norihiro Suzuki1,2, Minoru Osada3, Motasim Billah3,4, Yoshio Bando3,4, Yusuke Yamauchi5,6, Shahriar A. Hossain3,4 1Research Institute for Science and Technology (RIST), Tokyo University of Science (TUS), 2International Center for Young Scientists (ICYS), National Institute for Materials Science (NIMS), 3International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 4Australian Institute for Innovative Materials (AIIM), University of Wollongong, 5School of Chemical Engineering, Australian Institute for Bioengineering and Nanotechnology (AIBN), University of Queensland, 6Department of Plant & Environmental New Resources, Kyung Hee University Here, we present a protocol for the synthesis of porous barium titanate (BaTiO3) thin film by a surfactant-assisted sol-gel method, in which self-assembled amphipathic surfactant micelles are used as an organic template. Engineering Design and Evaluation of Smart Glasses for Food Intake and Physical Activity Classification Jungman Chung1, Wonjoon Oh2, Dongyoub Baek3, Sunwoong Ryu2, Won Gu Lee4, Hyunwoo Bang2 1School of Mechanical and Aerospace Engineering, Seoul National University, 2Envisible, Inc., 3Curiosis, Inc., 4Department of Mechanical Engineering, Kyung Hee University This study presents a protocol of designing and manufacturing a glasses-type wearable device that detects the patterns of food intake and other featured physical activities using load cells inserted in both hinges of the glasses. Behavior Visualizing Motion Patterns in Acupuncture Manipulation Ye-Seul Lee1, Won-Mo Jung1, In-Seon Lee1, Hyangsook Lee1, Hi-Joon Park1, Younbyoung Chae1 1Acupuncture and Meridian Science Research Center, College of Korean Medicine, Kyung Hee University Here, we present a protocol for using the Acupuncture Manipulation Education System (AMES) in the training of acupuncture manipulation skills using phantom acupoints. Bioengineering Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect Daniel S. Oh1, Alia Koch1, Sidney Eisig1, Sahng Gyoon Kim2, Yoon Hyuk Kim3, Do-Gyoon Kim4, Jae Hyuck Shim5 1Oral and Maxillofacial Surgery, Columbia University, 2Endodontics, Columbia University, 3Mechanical Engineering, Kyung Hee University, South Korea, 4Orthodontics, The Ohio State University, 5Pathology, Weill Cornell Medical College A step-by-step generic process to create a bone-like template with engineered micro-channels is presented. High absorption and retention capabilities of the template are demonstrated by capillary action via micro-channels.