Roskilde University 1 article published in JoVE Biology Optimized Scratch Assay for In Vitro Testing of Cell Migration with an Automated Optical Camera Michelle Vang Mouritzen1, Håvard Jenssen1 1Department of Science and Environment, Roskilde University Here we describe a procedure to test the cell migration in vitro with an automated optical camera microscope. The scratch assay has been widely used where the closure of the scratch is followed for a set period. The optical microscope enables dependable and cheap detection of cell migration.