University Hospital of North Norway 1 article published in JoVE Biology Analyzing Mitochondrial Morphology Through Simulation Supervised Learning Abhinanda Ranjit Punnakkal1, Gustav Godtliebsen2, Ayush Somani1, Sebastian Andres Acuna Maldonado3, Åsa Birna Birgisdottir2,4, Dilip K. Prasad1, Alexander Horsch1, Krishna Agarwal3 1Department of Computer Science, UiT The Arctic University of Norway, 2Department of Clinical Medicine, UiT The Arctic University of Norway, 3Department of Physics and Technology, UiT The Arctic University of Norway, 4Division of Cardiothoracic and Respiratory Medicine, University Hospital of North Norway This article explains how to use simulation-supervised machine learning for analyzing mitochondria morphology in fluorescence microscopy images of fixed cells.